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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Helicobacter pylori-induced NAT10 stabilizes MDM2 mRNA via RNA acetylation to facilitate gastric cancer progression

Fig. 7

Remodelin suppresses gastric cancer growth and improves the sensitivity of GC cells to MDM2 inhibitors. A and B Dose–response curves (A) and growth IC50 values (B) of gastric cancer and normal gastric cell lines following exposure to Remodelin for 72 h in a CCK-8 assay. C and D Clonogenic assay of gastric cell lines after exposure to increasing concentrations of Remodelin. E Nude mice were subcutaneously implanted with BGC823 cells and treated intraperitoneally with different doses of Remodelin daily for 4 weeks (5 mice per group). Tumor volumes were measured as a surrogate for tumor burden. F Survival curve of mice treated as described in E. G p53, p21 and MDM2 protein levels in AGS cells treated for 24 h with DMSO (vehicle), HDM201 (or Nutlin-3), Remodelin, or a combination of HDM201 (or Nutlin-3) and Remodelin were tested by Western blotting. Densitometric analysis for p53, p21, and MDM2 is shown in the right panel. H and I Combination study of the inhibitory effects of Remodelin and MDM2 inhibitors on the growth of the p53 wild-type GC cell lines AGS and BGC823 based on a CCK-8 assay. The combination index (CI) values were determined with CompuSyn software. J BGC823 cells were subcutaneously implanted into nude mice. Animals were treated daily with vehicle control, HDM201 (10 mg/kg), Remodelin (30 mg/kg), or the combination of both drugs for 4 weeks (6 mice per group). The tumor volume is shown. K Survival curves of mice treated in J. Rem, Remodelin; Nutlin, Nutlin-3. Error bars indicate the SD. *P < 0.05, **P < 0.01, ***P < 0.001(two-tailed t-test). ns, not significant

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