Fig. 2

Tumor growth in obesity-lymphoma mice. A Schematic diagram for establishing obese mouse model by WSHFD and establishing obesity-lymphoma model by EL4 cells subcutaneous xenograft in obese mice. B The body weight change after xenograft in EL4-WSHFD mice and EL4-CD mice, as well as the non-tumor controls. C The changes of tumor volume after lymphoma cell xenograft in EL4-WSHFD mice and EL4-CD mice. D The adipose tissue weight and tumor weight in EL4-WSHFD mice and EL4-CD mice measured at sacrifice. E The lymphoma staging according to Ann Arbor staging classification in EL4-WSHFD mice and EL4-CD mice. F Representative images of gross anatomy of harvested lymph nodes, histology by H&E, and Ki-67 staining by IHC in the tumor tissues from EL4-WSHFD mice and EL4-CD mice. For the histological details in H&E staining, the extensively distributed lymphoma cells were showing at least three to five times the size of the normal lymphocytes (arrow). The lymphoma cells had round nuclear outlines, vesicular chromatin, and single to multiple prominent nucleoli. For the Ki-67 staining, most Ki-67 positive cells are lymphoma cells (brown color). G Western blot analysis for the protein levels of cyclin D1, c-Myc, E-cadherin, and Vimentin in the tumor tissues from EL4-WSHFD mice and EL4-CD mice. H Triglyceride levels in the serum and tumor tissue from EL4-WSHFD mice and EL4-CD mice, as well as the non-tumor controls. *, P < 0.05; **, P < 0.01 ***, P < 0.001