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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: S1PR1/S1PR3-YAP signaling and S1P-ALOX15 signaling contribute to an aggressive behavior in obesity-lymphoma

Fig. 3

Lipid metabolism, S1P synthesis and S1P receptors in obesity-lymphoma mice. A Heat map of the key FFAs metabolic enzymes including FA transport (Slc27a1, Slc27a2 Slc27a3, Slc27a4, and CD36), FA oxidation (Acads, Acadm, Acox1, Cpt1a, and PPAR-α), export (Mttp and Apoa1), esterification (Dgat1 and Acat1) and FA synthesis (FASN, ACC1, ACC2, PPAR-γ and Srebp1) by q-PCR analysis in the tumor tissues from EL4-WSHFD mice and EL4-CD mice, as well as the non-tumor controls. B Heat map of the enzymes for S1P synthesis by q-PCR analysis in the tumor tissues from EL4-WSHFD mice and EL4-CD mice, as well as the non-tumor controls. C Schematic diagram of S1P biosynthetic cascade enzymes for the fold changes of WSHFD-EL4 mice versus CD-EL4 mice. ND, no detection; NC, no change; Red, > 2-fold up-regulated; Blue, < 2-fold down-regulated. D Western blot analysis for the protein levels of SPHK1 and phosphorylated SPHK1 in the tumor tissues from EL4-WSHFD mice and EL4-CD mice. E The levels of S1P production in the tumor tissues from EL4-WSHFD mice and EL4-CD mice. F Western blot analysis for the protein levels of the S1P receptors, S1PR1 and S1PR3. G Representative images of IHC staining for S1PR1 and the computer-imaging analysis of S1P expression levels in the tumor tissues from EL4-WSHFD mice and EL4-CD mice. X20, 200-fold magnification. *, P < 0.05; **, P < 0.01

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