Fig. 4

The S1P-S1PR1/S1PR3-YAP signaling. A, B Western blot analysis for the protein levels of phosphorylated YAP in the tumor tissues from mice and in the lymphoma cells treated with S1P. C Schematic diagram of S1P/S1PR1 mediated YAP signals. D Western blot analysis for the protein levels of phosphorylated YAP in the lymphoma cells treated with S1P and inhibitors of S1P receptors. E, F A XTT cell viability assay and a trans-well assay as well as Wester blotting for cell proliferation and migration of the lymphoma cells treated with S1P and inhibitors of S1P receptors. G Western blot analysis for the protein levels of E-cadherin and Vimentin for EMT, CTGF for YAP targeting gene, and cyclin D1 for cell cycle progression in the lymphoma cells treated with S1P and inhibitors of S1P receptors. *, P < 0.05; **, P < 0.01; ***, P < 0.001