Fig. 5

TAMs and macrophage polarization in obesity-lymphoma mice. A In the tissues of the tumor invaded lymph node from xenograft model of EL4-WSHFD mice and EL4-CD mice, immunofluorescent staining was performed using the antibodies of anti-CD11b and anti-Ly6C as well as the antibodies of anti-CD206 and anti-F4/80 to detect the M-MDSC derived macrophages. Green: positive staining for CD11b or CD206; red: positive staining for Ly6C or F4/80; blue: positive DAPI (4′,6-diamidino-2-phenylindole) staining to detect the nuclei as a counterstain. B Flow Cytometry analysis to detect CD11b⁺Ly6C⁺ cells and F4/80⁺CD206⁺ cells in the collected PBMC and PMM from peritoneal injection model of EL4-WSHFD mice and EL4-CD mice. C q-PCR analysis of M2 phenotype in the collected PMM from peritoneal injection model of EL4-WSHFD mice and EL4-CD mice. D Schematic diagram of PMA induced M0 phenotype, LPS induced M1 phenotype, and S1P induced M2 phenotype in THP-1 monocytes. E Western blot analysis for the protein levels of arginase-1, TGFβ, and ALOX15 in the PMA induced M0 THP-1 monocytes treated with LPS and/or S1P. HPF: high-power field; PMA: phorbol 12-myristate 13-acetate; PMM: peritoneal monocytes/macrophages. Scale bar = 100 μm. *, p < 0.05; **, p < 0.01