Fig. 2

The top 2 abundant infiltrating immune cells were tumor-associated macrophages and T cells. (A) Schematic of the study design. Single-cell RNA sequencing was used to analyze infiltrating immune cells isolated from tumor-bearing mice. (B) Clustering of intratumoral CD45⁺ cells and visualization of the subset. Uniform manifold approximation and projection (UMAP) of single-cell RNA sequencing data from 6563 cells (Control: 3387; Drug: 3176; upper) revealed six clusters determined by 14 specific markers (Supplementary Fig. 3). Each dot plot represents one cell. (C) Bubble heatmap determining the expression levels of specific marker genes in the CD45⁺ subsets and fibroblasts. (D) The percentages of CD45⁺ subsets and fibroblasts in the control and low-dose SNAP treatment groups