Fig. 1

PDOs recapitulate morphologic features of original tissue and can be obtained from low cellularity biopsies. (A) Culture images of some PDOs lines (top panel; scale bar 50 μm, 20X), compared with corresponding H&E staining (hematoxylin and eosin) (middle panel; scale bar 50 μm, 20X) and with matched tissue stained with H&E (bottom panel; scale bar 100 μm, 10X). (B) Corresponding percentage of tumor cells assessed as percentage of neoplastic cells with respect to the total amount of viable cells in the tissue sample from which the culture was derived, assessed by the pathologist. (C) IHC for CDX2 and CK20 antibodies in a selection of PDOs. CNO75 is a normal mucosa organoid from a CRC patient, used as control for both markers. (Scale bar 50 μm, 20X). (D) Lack of expression of MLH1 and PMS2 in a core biopsy from a liver metastasis of patient 50 and its corresponding derived PDOs. RTO2 is a pMMR model used as positive control. (Scale bar 50 μm, 20X). T: tissue