Fig. 3

PDZK1IP1 suppression promotes OSCC cell migration and metastasis in vitro and in vivo. A-D OEC-M1 cells transfected with shMAP17-140 and shMAP17-185 for silencing endogenous PDZK1IP1 expression (and controls) (A, B) and their measured migration ability (C, D). E–H mRNA (E) and protein (F) expression of SAS cells transfected with PDZK1IP1 overexpression plasmids and their migration ability, as determined through a wound-healing assay (G, H). I Western blotting of OEC-M1 cells transfected with shMAP17 and SAS cells transfected with PDZK1IP1 overexpression plasmids. J In the animal model experiment, IVIS imaging was used to detect luciferase signals in the group with PDZK1IP1-knockdown OEC-M1 cells and the control group. Lower panel, the radiance range was reduced (from 2.2e4-2.8e6 to 1e4-3.4e4) to see low-intensity images of some mice. K Luciferase signals detected using an IVIS imaging system from week 1 to week 5. *p < 0.05 by Mann–Whitney U Test. L Metastatic lung nodules were retrieved after mice were euthanized. Hematoxylin and eosin staining was used to detect tumor parts and IHC staining was used to detect MAP17. M Calculated metastatic lung nodules after mice were killed. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by Student’s t test