Fig. 4

PDZK1IP1 suppression promotes OSCC migration by regulating TGF-β signaling. A, B Wound-healing assays of OEC-M1 cells transfected with shMAP17 and controls, treated with TGF-β inhibitor or not. C TGFβ1 mRNA expression of OEC-M1 cells transfected with pLe-miR-455-5p (or controls). The difference was increase after cells treated with TGF-β1 (10 ng/ml, #100–21, PeproTech). D Smad-binding element luciferase reporter assay of OEC-M1 cells transfected with shMAP17. E Western Blot analysis exhibited nuclear and cytoplasmic fractionation of phosphor-Smad3 (p-Smad3) or Smad4. F-G Box plots indicated fold change of p-Smad3 or Smad4 in nuclear and cytoplasmic fractionation of OEC-M1 cells transfected with pLe-miR-455-5p (or controls) (F) or transfected with shMAP17 (or controls) (G)