Fig. 6

The function of NK cells was suppressed in the TME of CCII patients. (A) The hierarchical clustering of the top 50 DEGs of NK cells between the CCI and CCII groups. (B) The distribution of the NK cells expressing GZMA, GZMB, NKG7, and KLRC1 in a 2D-tSNE space. (C) The comparison of the average expression of the genes associated with chemokine and cytokine signalling with respect to their percentage of expression in the two groups. (D) The ranking of the DEGs supporting the enrichment of natural killer cell mediated cytotoxicity pathway in the CCI group, with respect to the CCII group. (E) The 2D-tSNE graphs show the distribution of NK cells of the two groups and the subtypes, as well as the proportions of each subtype in individual patient. (F) Pseudotime trajectory analysis of NK cells. (G) The seven states identified along the pseudotime and their composition of the subtypes of NK cells. (H) Comparison of the normalised expression of the marker genes in each state of the two group, including GZMA, GZMB, NKG7, KLRC1, CCL5 and CCL4I