Fig. 1

A CRISPR-based dropout screen identifies sensitivities for each of the CMS of CRC. A. Schematic overview of set-up CRISPR-based drop-out screen. B. Table of the cell lines used in the drop-out screen representing each of the CMS. C. Bubble plots represent outcome of MAGeCK analysis comparing, clockwise, CMS1 to CMS2–4, CMS2 to CMS1, 3, 4, CMS3 to CMS1, 2, 4 and CMS4 to CMS1–3 CRC cell lines. Each bubble represents one kinase, listed randomly. All significant CMS-specific drop-outs are highlighted in color. Negative false discovery rate for MAGeCK output was set to 0.01. Bubble size represents calculated median fold change for all sgRNAs targeting the same gene. FC = Fold change. D, E. Heatmaps depicting Z-score transformed 2Log gene expression of the top 2 most significant drop-outs for each CMS in cell lines (D) and CRC tumor samples (E). The color of the labeled kinases indicates the subtype for which it significantly drops-out, as is depicted in the legend