Fig. 5

CMS4 cell lines depend on PAK2 for invasion and metastasis. A, B. Representative images (A) and quantification (B) of Matrigel-coated Transwell experiments. Bar plots display relative area covered by cells (purple) in Transwell image. FC = fold change relative to WT mean value. Mean + S.D. derived from N = 5 are plotted. * = P < 0.05 in one-tailed Mann-Whitney test. C. Illustration indicates regions scored for abundance of metastatic foci in the intraperitoneal metastasis mouse model. Maximum score per region is 3 and thus 21 in total. D. Bar graphs depict mean PCI score + s.e.m. Individual dots indicate score for one mouse. ** = P < 0.005, * = P < 0.05. E. Schematic drawing of mesenteric vein injection assessing capacity to colonize liver and secondary spread to lungs. F-I. Macroscopic images (F, H) of tumor burden in full liver and lungs taken directly after sacrificing mice injected with HuTu-80 WT-Dendra (green) and PAK2-KO-tdTomato (magenta) cells. Colonization of organs and number of metastases was quantified through immunohistochemistry for human Ku80 on three liver sections and 1 lung section per mouse (G, I). Bar graphs indicate mean + s.e.m. * = P < 0.05, *** = P < 0.001 as calculated by one-tailed, unpaired Student’s T-test. Scale bar represents 500 μm. Each colored bubble indicated on G, I represents an individual mouse used in the experiment