Fig. 5

The activities of LPPC/DC MP complexes. A The picture illustrated how to prepare the LPPC/MP complexes to treat mice splenocytes in vivo. B The cell proliferation rates of splenocytes from different treatments were evaluated by the MTT assay. Microgram was abbreviated as mcg. C, D The splenocytes from different treatments were pulsed with antigens, such as IFN-γ and IL-4, and cytokine secretion at different times was estimated by ELISA. A significant difference compared to the LPPC group is indicated by * (P < 0.05) and ** (P < 0.01). Each data indicated the mean ± SD from three independent experiments (N = 6). E The numbers of IFN-γ or IL-4 expressing T cells responding to HpHSP60 in treated mice were evaluated by an ELISPOT kit. The spots were observed, and the data were calculated and expressed as the mean ± SD (N = 6, *: P < 0.05)