Fig. 2
LARP6 inhibits CRC cell invasion and metastasis in vitro and in vivo. A-B Identification of LARP6 overexpression (B) and interference (A) effects through qPCR and WB. C-D Transwell migration assays in CRC cells with LARP6-overexpression (D) or knockdown (C). Statistical results are shown in right panels (N = 3). E-F Marigel transwell invasion assays of LARP6-overexpression (F) or LARP6-knockdown (E) in CRC cells. Statistical results are shown in right panels (N = 3). G Representative images of orthotopic CRC mice model constructed with LARP6-interfered CACO2 cells or control cells: fluorescence in vivo imaging at 60 days after injection (left), macroscopic pictures of colon and liver samples (middle), and H&E staining of liver tissues and primary colon tumor tissues (right). The blue arrow indicates the tumor. H Summary results of the nude mice presenting with liver metastasis between LARP6-knockdown group and control group. I The primary tumor growth was measured by volume (cm3). *P < 0.05, **P < 0.01, ***P < 0.001, ns means no statistic difference. The error bars represent Mean ± SD