Fig. 5

By up-regulating ZNF267 expression, LARP6 suppresses SGMS2 expression. A-B Relation between LARP6 and sphingolipid metabolism in KEGG enrichment analysis. Sample information were from three different GEO datasets as before. NES: normalized enrichment score. C qPCR was used to examine CNOT2 and SGMS2 mRNA expression in SW480 cells upon LARP6 over-expression. D Correlation analysis of LARP6 and SGMS2 expression in GEO datasets. r value represents correlation. E-F ZNF267 inhibited the transcription of SGMS2: (E) CHIP-qPCR and Dual-luciferase reporter gene assay (F) verified the transcription inhibition of SGMS2 by ZNF267. G The suppression of ZNF267 on SGMS2 protein expression was shown through WB. H-M After interference or ectopic expression of ZNF267, qPCR (H-K) and WB (L-M) were experimented to detect SGMS2 expression in LARP6 overexpression or knockdown cells. *P < 0.05, **P < 0.01, ***P < 0.001, ns means no statistic difference. The error bars represent Mean ± SD