Fig. 6

LARP6 inhibits SGMS2-mediated sphingomyelin synthesis. A-D Ceramide ELISA Kit and sphingomyelin Kit were used to detect the total ceramide (A, C) and sphingomyelin (B, D) level in CRC cells with LARP6 overexpression or interference (N = 3). E-I In LARP6-overexpressed or interfered CRC cells, total ceramide and sphingomyelin level were detected after forced expression or knockdown of ZNF267 or SGMS2: E identification of overexpression or interference of ZNF267 and SGMS2 effect, F-I total ceramide and sphingomyelin level detection by ceramide ELISA Kit and sphingomyelin Kit (N = 3). J-M With expression restoration of ZNF267 or SGMS2 and the use of Ly93 inhibitor, matrigel transwell assays in LARP6-knockdown CRC cells were conducted (J-K). Statistical results were shown below (L-M) (N = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ns means no statistic difference. The error bars represent Mean ± SD