Fig. 7

LARP6 upregulates autophagy activity in CRC cells. A With or without BafA1, protein expression of LC3B-II and P62 were examined by WB in LARP6-downregulated CRC cells. B-C Electron microscopy images presenting the ultrastructure of the CRC cells after LARP6 knockdown. Yellow arrows indicate autophagic vesicles. Statistical results are shown below (N = 3). E-F After transfected with RFP-GFP-LC3B lentivirus, autophagy flux in CRC cells with LARP6 interference were evaluated using a confocal microscope by quantitation of the number of red and yellow puncta in cells, counting at least 10 cells per group. Red dots indicate autophagolysosomes, and yellow dots represent autophagosomes. Statistical analysis are shown on right pannel (E) (N = 3). D and G With the presence of Ly93 inhibitor, protein expression of LC3B-II and P62 were examined (D), and the autophagic flux (G) was monitored in SW620 cell with stable LARP6 knockdown. Statistical analysis are shown on right pannel (N = 3). H Matrigel transwell detection in LARP6-knockdown CRC cells with Rapa (100 nM) treatment for 24 h. Statistical results are shown on the right (N = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ns means no statistic difference. The error bars represent Mean ± SD