Fig. 8

LARP6 suppresses CRC metastasis through ZNF267/SGMS2 axis. A-D Analysis of primary CRC tissues in orthotopic CRC mice model constructed with LARP6-interfered CACO2 cells or control cells: IHC staining of LARP6, ZNF267 and SGMS2 (A), total ceramide (B) and sphingomyelin (C) level examination using ceramide ELISA Kit and sphingomyelin Kit, and autophagy detection with LC3B-II and P62 protein (D). E-F With the use of Ly93 inhibitor and Rapa, in vivo rescue experiment was conducted. (E) Representative images of orthotopic CRC mice model: fluorescence in vivo imaging at 60 days after injection (left) and H&E staining of liver tissues and primary colon tumor tissues (right). (F) Summary results of the nude mice presenting with liver metastasis between groups of in vivo rescue experiment. G-H Correlation analysis of LARP6 with ZNF267 and SGMS2 expression in 165 paraffin-embedded primary CRC tissues and matched adjacent normal tissues. I Schematic diagram of our research representing the function and potential mechanism of LARP6 in CRC. *P < 0.05, **P < 0.01, ***P < 0.001, ns means no statistic difference. The error bars represent Mean ± SD