Fig. 2

GPX8 is involved in ccRCC cell growth in vitro and in vivo A-D, The effect of GPX8 KO in Caki1 and shGPX8 in 786O cells in vitro. Western blot analysis of GPX8 protein expression (A), relative growth rates measured by CCK8 kit (B), migrating cells in scratch assay at 0, 24, and 48 h (top) and bar graphs for relative wound areas for WT and GPX8-KO Caki1 cells (bottom) (n = 3) (C), and clonogenic assay after plating 200 cells in 6-well plate for 2 weeks (top) and bar graphs for number of colonies of WT and GPX8-KO Caki1 (bottom) (n = 3) (D). E, Tumor growths by WT and GPX8-KO Caki1 cells xenografted in nude mice (n = 6). One mouse in the GPX8-KO group did not develop a visible tumor, and the tumor photo shows 5 tumors. See Fig. S2C for the photos for the whole body mouse images. Tumor volume (top, left), tumor weight (top, right), and photograph of tumors (bottom) obtained at day 28 after implantation. Data presented in panels (B), (C), (D), and (E) are means ± SD (n ≥ 3). P-value was calculated by two-way ANOVA with Geisser–Greenhouse correction for (B) and (E) (top, left panel); unpaired t-test for (C), (D) and (E) (top, right panel)