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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Integrated multi-dimensional analysis highlights DHCR7 mutations involving in cholesterol biosynthesis and contributing therapy of gastric cancer

Fig. 3

Knockdown of DHCR7 inhibited the proliferation, invasion and migration of GC cells and promoted apoptosis. A Protein expression of DHCR7 was signicicantly upregulated in AGS, MKN-45, MKN-28 and downregulated in HGC-27 cells. B Western blot analysis confirmed that DHCR7 expression was knocked down by siRNAs in GC cell lines. C Proliferation of AGS and MKN-28 cells with different DHCR7 expression levels, as determined by a CCK-8 assay at different time points over a 96 h period. D Results of EdU incorporation assays in AGS and MKN-28 cells transfection with DHCR7 siRNAs. E Results of colony formation assays in AGS and MKN-28 cells transfection with DHCR7 siRNAs. F Results of Transwell migration and invasion assays in AGS and MKN-28 cells transfection with DHCR7 siRNAs. G Results of the apoptosis assay in AGS and MKN-28 cells transfection with DHCR7 siRNAs. The data are presented as the means ± SDs and calculated by Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001

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