Fig. 5

Overexpressing DHCR7 increased the cholesterol level and further promoted the malignant phenotype of GC in vivo. A Results of xenograft tumor model generated with DHCR7-overexpressing GC cell and control GC cell (n = 5 per group). B Representative images of IHC for Ki-67 in tumor tissues from OE-DHCR7 and OE-NC groups. C Results of tissue cholesterol detection in tumors from OE-DHCR7 and OE-NC groups. D Results of murine lung metastasis model generated with DHCR7-overexpressing GC cell and control GC cell (n = 4 per group). E Representative images of HE stains in lung metastasis from OE-DHCR7 and OE-NC groups. F Results of TAM treatment on DHCR7-overexpressing GC cell in xenograft tumor model (n = 5 per group). G Representative images of IHC for Ki-67 in tumor tissues from OE-DHCR7 and OE-DHCR7-TAM groups. H Weight of tumors from OE-DHCR7 and OE-DHCR7-TAM groups. I Tissue cholesterol level of tumors from OE-DHCR7 and OE-DHCR7-TAM groups. J Results of TAM treatment on DHCR7-overexpressing GC cell in murine lung metastasis model (n = 5 per group). K Representative images of HE stains in lung metastasis from OE-DHCR7 and OE-DHCR7-TAM groups. The data represent the means ± SEMs and calculated by Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001