Fig. 7From: Integrated multi-dimensional analysis highlights DHCR7 mutations involving in cholesterol biosynthesis and contributing therapy of gastric cancerMutant DHCR7 inhibited the proliferation, invasion and migration of GC cells and promoted apoptosis. A The proliferation of GC cells with WT/MT DHCR7 was detected by an EdU incorporation assay. B Results of colony formation assays in GC cells with WT/MT DHCR7. C Results of Transwell migration and invasion assays in GC cells with WT/MT DHCR7. D Results of the apoptosis assay in GC cells with WT/MT DHCR7. E Cholesterol level in GC cells with WT/MT DHCR7. F Cellular cholesterol level, as measured by Filipin III staining. The data are presented as the means ± SDs and calculated by Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001Back to article page