Fig. 2

The immune landscape and prognostic significance of ESCC tumor microenvironment. (A) UMAP visualization of 41,237 cells from 22 peri- and tumor samples (PRJNA777911). (B) Heat map of signature matrix genes derived by CIBERSORTx from scRNA-seq data distinguishing 10 cell types. (C) Heatmap of the normalized absolute abundance for each cell type in the ESCC microarray cohort revealed distinct microenvironment compositions between peri- and tumor sites with t-test. (D) Kaplan–Meier curves of overall survival of ESCC patients stratified by the abundance of macrophages (left), fibroblasts (middle) and mast cells (right)