Fig. 6

Identification of a CCL18 peptide inhibitor. (A) Sequence alignment of human CCL18, human and mouse CCL3 by the online tool ESPript3. The protein structure was labeled according to CCL18. The consensus sequences were listed. The numbers in green represent the cysteine residues to form the disulfide bonds. (B) The structure of human CCL18, human and mouse CCL3 were superposed as the sequence alignment using the software MOE. (C) The information of peptides derived from human CCL18 or CCL3 selected based on the structure. The effects on the proliferation of EC-109 cells transfected with the vector or shPITPNM3 with or without the existence of 20 ng/mL rhCCL18 by Pep1 (D), Pep2 (E), Pep3 (F) and Pep4 (G) in indicated concentrations using the MTT assay. *P < 0.05, **P < 0.01, ***P < 0.001