Fig. 1

Identification and characterisation of circFAM13B in BCa. A Heat map of circRNA sequencing in five pairs of BCa tissues. B Schematic illustration that shows that circFAM13B was composed of FAM13B exon 8, exon 9 and exon 10, and the back splicing junction was confirmed by sanger sequencing. C The expression of circFAM13B and FAM13B mRNA in T24 and UMUC3 cells treated with or without RNase R were determined by qRT-PCR (*P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test). D The remaining RNA levels of circFAM13B and FAM13B mRNA in T24 cells treated with actinomycin D at different time points were determined by qRT-PCR (*P < 0.05, Student’s t-test). E The expression of circFAM13B in cDNA and gDNA of T24 cells were confirmed by qRT-PCR with the divergent and convergent primers. F The subcellular location of circFAM13B was validated by FISH experiment. G The expression of circFAM13B in seven BCa cell lines and SV-HUC cell line were investigated by qRT-PCR (**P < 0.01, ***P < 0.001, Student’s t-test). H The expression of circFAM13B in 72 pairs of BCa tissues was confirmed by qRT-PCR (**P < 0.01, Student’s t-test). I The relationship of circFAM13B and the overall survival of patients with BCa was confirmed by Kaplan–Meier analysis. Data are expressed as mean ± SD, n = 3