Fig. 6

IGF2BP1 promoted the glycolysis and immune escape of BCa cells. A-B Glucose, lactic acid and ATP detection assays were performed in IGF2BP1 siRNAs transfected or control T24 and UMUC3 cells (*P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test). C–D ELISA assays were carried out to detect the granzyme B and IFN-γ produced by CD8⁺ T cells, which were co-cultured with IGF2BP1 siRNA transfected or control T24 and UMUC3 cells (**P < 0.01, ***P < 0.001, Student’s t-test). E–F The killing ability of CD8.⁺ T cells and the immunotherapy sensitivity of BCa were increased when co-cultured with IGF2BP1 knockdown T24 or UMUC3 cells. Data are expressed as mean ± SD, n = 3