Fig. 8

CircFAM13B overexpression augments the efficacy of anti PD-1 therapy in HuNOG mice model. A Schematic of the huNOG mice model establishment and experimental design to investigate the role of circFAM13B in regulating immune escape and anti-PD-1 therapy efficacy in vivo. B Flow cytometry was performed to detect the positive rate of human CD45 in the HuNOG mice model. C Representative image of HuNOG mice injected with circFAM13B or relative control T24 cells, which were treated with PD-1 or IgG antibodies (n = 5). D Representative image of the tumour formation of HuNOG mice injected with circFAM13B or relative control T24 cells, which were treated with PD-1 antibodies or (n = 5). E The weights of the tumours removed from HuNOG mice were measured using electronic scales (**P < 0.01, ***P < 0.001, Student’s t-test). F The tumour volumes of HuNOG mice were measured every 3 days (***P < 0.001, Student’s t-test). G Representative images of IHC staining of Ki-67, PKM2, CD8 and CD3 in tumour samples removed from HuNOG mice. Data are expressed as mean ± SD, n = 3