Fig. 5

A In the LCN2-knockdown group, after treatment with TGF-α, EGFR activation was significantly increased after 2 h and quickly dissipated after 4 h, while the results in the control group were the same as those in the ctrl-OV control group, showing activation after 1 h and dissipation after 4 h. B p-EGFR gray value statistics. After inhibiting the expression of LCN2, p-EGFR appeared slowly but dissipated rapidly. C In LCN2-overexpressing cells, after TGF-α treatment, p-EGFR was rapidly activated in the LCN2-OV group and gradually dissipated after 4 h, but p-EGFR in the control group cells was only activated after 1 h and dissipated after 4 h. D p-EGFR gray value statistics. p-EGFR quickly appeared and slowly dissipated after LCN2 was upregulated. E After the expression of LCN2 was inhibited, a small amount of EGFR was transferred from outside of the cell membrane to the inside of the cell after TGF-α stimulation, but the degree of transfer was less than that of the control group, and there was no significant difference. (Scale bar: 20 μm). F IF analysis showed that after LCN2 overexpression, EGFR localization rapidly shifted from an extramembranous to an intracellular location after TGF-α treatment, and colocalization with LCN2 increased, while in the control group, EGFR was still mostly located on the cell membrane, and a small amount entered the cells to colocalize with LCN2. (Scale bar: 20 μm)