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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Silencing LCN2 suppresses oral squamous cell carcinoma progression by reducing EGFR signal activation and recycling

Fig. 7

A Schematic diagram showing the structure of mPEG-SS-PLGA nanoparticles and their release of siLCN2 in cells through lysosomal escape and the response to glutathione. B Electron microscopy images showing the particle size of the nanoparticles and their release in the presence of 20 mM glutathione. (Scale bar: 200 nm). C After the nanoparticles encapsulated siLCN2, the release of siLCN2-Cy5 in the presence of glutathione (20 mM) was monitored. At a glutathione concentration of 20 mM, more siLCN2 was released. D Nanoparticle size detection; the nanoparticle size was between 80–120 nm. E Nanoparticle potential detection; the potential was approximately -20 mV. F OSCC cells were transfected with RNAiMAX transfection reagent and nanoparticle transfection reagent. Western blotting showed that both transfection methods significantly inhibited the expression of LCN2. G After LCN2 expression was downregulated in OSCC cells, the migratory abilities of the cells were significantly reduced. (Scale bar: 100 μm). H The scratch healing assay showed that after LCN2 expression was downregulated in CAL-27ER and HN-6ER cells by NPs, cellular migration was decreased. I The cell colony formation test showed that the colony formation of OSCC cells decreased significantly in the NPs-siLCN2 groups. J and K Inhibition of LCN2 in OSCC cells by NPs significantly decreased their proliferation abilities, and the CCK-8 results were lower than those of the control group

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