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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Exosomal circTUBGCP4 promotes vascular endothelial cell tipping and colorectal cancer metastasis by activating Akt signaling pathway

Fig. 3

CircTUBGCP4 in CRC-CDEs was associated with angiogenesis and metastasis in CRC. a The percent of different circRNA types in HCT11-Exo and serum-Exo, each point represents 1%. b Venn diagram of circRNA identified in HCT11-Exo and serum-Exo. c The volcano map of differently expressed circRNA in serum-Exo from CRC patients with metastasis or non-metastasis. p < 0.05, fold change > 1.5 or fold change < -1.5. d The screen flow of circRNA by three databases that circRNA in HCT116-Exo, Top 20 up-regulated circRNA in serum-Exo, and exoRBase. e The expression of circ104109 and circ101501 in HUVECs, HCT116, and SW480. GAPDH was the internal control. f The detail of circTUBGCP4 and Sanger sequence of amplification products using a divergent primer. g Agarose gel analysis of PCR production using divergent primer and convergent primer of circTUBGCP4. h qPCR analysis of circTUBGCP4 and TUBGCP4 expression after HCT116 cells culture with actinomycin D at 0, 4, 8, 12, and 16 h. GAPDH was the internal control. i GSE126094 reanalysis of circTUBGCP4 expression in CRC T compared with AN. j CircTUBGCP4 expression in CRC LM compared with CRC non-LM using GSE147597 data. k ISH analysis of circTUBGCP4 in the clinical cohort from 20 pairs CRC T(M) and AN. Scale bars = 50 μm. l Correlation analysis between circTUBGCP4 expression and CD31, CD34 expression in tumor tissue. Student's t-test, *P < 0.05, 0.001 < ** P < 0.01, *** P < 0.001

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