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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Exosomal circTUBGCP4 promotes vascular endothelial cell tipping and colorectal cancer metastasis by activating Akt signaling pathway

Fig. 5

Overexpressed circTUBGCP4 promotes angiogenesis and tumor metastasis. a A flowchart of lung metastasis model building using exosome derived from HCT116- ShCirc stable cell lines in vivo. Exosomes (40 μg) are injected every 4 days for 12 days. PBS was used as a negative control. b Lung metastasis nodules in HCT116EXO-Sh-NC (116EXO-Sh-NC) groups, HCT116EXO-Sh-CircTUBGCP4-02 (116EXO-Sh-Circ-02) groups compared to PBS groups using HE staining. Scale bars = 200 μm. c The number of metastasis nodules was counted. d Blood vessels in lung metastasis nodule (red arrows) using HE staining. Scale bars = 100 μm. e The number of blood vessels (per nodule) was counted. f The efficiency of overexpressed circTUBGCP4 in HCT116 stable cell line. g Flow chart of lung metastasis model building using LV-NC HCT116 cells and LV-CircTUBGCP4 HCT116 cells in vivo. h In vivo fluorescence imaging of the metastasis model at 30 days. i Lung metastasis nodules in LV-CircTUBGCP4 compared to LV-NC using HE staining. Scale bars = 200 μm. The number of blood vessels (per nodule) was counted. j Blood vessels in lung metastasis nodule (red arrows) using HE staining. Scale bars = 100 μm. The number of blood vessels (per nodule) was counted. k and (i) Immunohistochemistry analysis of CD31 expression and CD34 expression in lung metastasis nodule, Scale bars = 20 μm. Mean ± SEM. Student's t-test, *P < 0.05, 0.001 < ** P < 0.01, *** P < 0.001

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