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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Targeting tumor exosomal circular RNA cSERPINE2 suppresses breast cancer progression by modulating MALT1-NF-𝜅B-IL-6 axis of tumor-associated macrophages

Fig. 1

Analysis of SERPINE2-derived circRNAs in breast cancer. a Volcano plot showing the differentially expressed circRNAs in breast cancer tissues compared to matched adjacent tissues in GSE182471 dataset. b Venn diagram showing the intersection of differentially expressed circRNAs in GSE182471 dataset and SERPINE2-derived circRNAs. c The relative expression of cSERPINE2 in breast cancer tissues and matched adjacent tissues (n = 18). d, e 136 breast cancer patients were divided into different groups according to tumor size or lymph node metastasis status. The diagram showing cSERPINE2 expression in each group. f Kaplan–Meier analysis of the OS and RFS of breast cancer patients according to cSERPINE2 expression. g Diagram illustrating back-splicing of cSERPINE2 from the host gene SERPINE2. h Representative images of FISH analysis of cSERPINE2 in MCF-7 and MDA-MB-468 cells. Scale bar, 5 μm. i The relative expression of cSERPINE2 in MDA-MB-468 cells after knocking down DHX9, QKI, ADAR-1, EIF4A3, FUS or HnRNPM. j Putative binding sites of EIF4A3 in cSERPINE2 pre-mRNA predicted by CircInteractome database (upper), and RIP assay (lower) of interaction of EIF4A3 with cSERPINE2 pre-mRNA in MDA-MB-468 cells. The H19 lncRNA was used as the positive control. k A schematic diagram of five fragments of cSERPINE2 pre-mRNA (upper), and RNA pull-down analysis (lower) of the interaction between EIF4A3 and the above five fragments of cSERPINE2 pre-mRNA. l Representative images (left) of ISH analysis of cSERPINE2 expression and IHC analysis of EIF4A3 expression in breast cancer tissues, and a correlation analysis (right) between the cSERPINE2 and EIF4A3 expression. Scale bar, 50 μm. Data are presented as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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