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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Targeting tumor exosomal circular RNA cSERPINE2 suppresses breast cancer progression by modulating MALT1-NF-𝜅B-IL-6 axis of tumor-associated macrophages

Fig. 5

Tumor exosomal cSERPINE2 promoted the secretion of IL-6 in TAMs via activating the NF-\(\upkappa\)B pathway to encourage progression of breast cancer. a ELISA assay of IL-6 concentration in the supernatants from BMDMs and mTAMs as indicated treatments. b Western blotting was performed to evaluate the expression levels of MALT1, phosphorylated IKK-β, IKK-β, phosphorylated IκBα and IκBα in BMDMs and mTAMs as indicated treatments. c p65 nuclear translocation in BMDMs and mTAMs as indicated treatments was assessed by immunofluorescent confocal microscopy. Scale bar, 5 μm. d Representative bioluminescence images (left) and quantification (right) of cSERPINE2 silenced and overexpressed tumor cells derived orthotopic breast cancer model after IgG or anti-IL-6 Abs treatments (n = 6 mice for each group). e Representative lung images (left) and quantification of metastatic foci (right) of cSERPINE2 silenced and overexpressed tumor cells derived orthotopic breast cancer model after IgG or anti-IL-6 Abs treatments (n = 6 mice for each group). f Representative IHC images (left) and quantification (right) of Ki67, CD44 expression and F4/80+ macrophages infiltration in cSERPINE2 silenced and overexpressed tumor cells derived orthotopic breast cancer model after IgG or anti-IL-6 Abs treatments. Scale bar, 50 μm. g, h Orthotopic breast cancer models were injected exosomes derived from EO771 cells with or without cSERPINE2 overexpression, followed by treatment of IgG or anti-IL-6 antibody (n = 6 for each group). Tumor size was measured over time (g). Representative H&E images (left) and quantification (right) of lung metastases (h). Scale bar, 100 μm. Data are presented as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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