Fig. 2
IGF1R inhibition enhanced the sensitivity of OSCC to DDP in vivo and in vitro. A Effect of shRNAs-IGF1R in ECA109 and KYSE150 cells at mRNA and protein levels under O2-poor mircoenvironment. B Proliferation ability was assessed by CCK-8 assay in OSCC cell lines transfected with shRNAs-control/IGF1R under hypoxia. C Representative images of tumours in a subcutaneous tumour model of nude mice injected with control/shIGF1R. D Weight (left) and volume (right) of subcutaneous tumour. Abbreviation: KD, knockdown. E Expression of IGF1R at the protein level in tumours of nude mice tested by WB and IHC. F IC50 of linsitinib in OSCC cell lines under hypoxic conditions. G The CI values of DDP at different concentrations combined with linsitinib in constant ratio, calculated by using the Chou–Talalay method. H Proliferation ability in OSCC cells treated with control/shIGF1R/linsitinib (linsitinib: ECA109, 5uM; KYSE150, 20uM) combined with DDP (ECA109, 2.5uM; KYSE150, 5uM). I Mice were intraperitoneally injected with DDP combined with linsitinib or not. Representative images of tumours from nude mice at day 28 are shown. J Weight (left) and volume (right) of subcutaneous tumour. Student’s t test. NS: not signifcant, *p < 0.05, **p < 0.01, ***p < 0.001