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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Targeting IGF1R signaling enhances the sensitivity of cisplatin by inhibiting proline and arginine metabolism in oesophageal squamous cell carcinoma under hypoxia

Fig. 5

IGF1R regulates the expression of ASS1 and PYCR1 through its signaling pathway–c-MYC axis under hypoxia. A Correlation between IGF1R expression and PYCR1 or ASS1 expression in OSCC tissues obtained from TMA analysis. B Protein levels of ASS1 and PYCR1 in OSCC cells transfected with siRNA-control/IGF1R cultured under limited-oxygen conditions. C Protein levels of RAS/MAPK and JAK/STAT in OSCC cells transfected with shRNA-control/IGF1R. D Protein levels of ASS1 and PYCR1 in OSCC cells transfected with siRNA-control/JAK (left) or siRNA-control/ERK (right). E Protein levels of ASS1 and PYCR1 in OSCC cells transfected with siRNA-control/c-MYC. F Protein levels of c-MYCT58 in OSCC cells transfected with siRNA-control/JAK (Top) or siRNA-control/ERK (Bottom). G, H, I Predicted sequences of c-MYC motifs (G) at the ASS1 (H) and PYCR1 (I) promoters determined using JASPAR. J, K ChIP-PCR analysis of c-MYC enrichment at the ASS1 (J) and PYCR1 (K) promoters. L, M Schematic diagram of luciferase reporter plasmids, which were constructed containing the wild ASS1(L)/PYCR1(M) promoters or mutant c-MYC motif. N, O Transactivation activity of indicated ASS1 (N) and PYCR1 (O) promoters in KYSE150 cells from luciferase reporter gene assays. P Protein levels of cMYC, c-MYC.T58, ASS1 and PYCR1 in OSCC cells transfected with siRNA-control/IGF1R cultured in limited oxygen. Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001

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