Fig. 6
The role of ASS1 and PYCR1 in OSCC. A, C Effects of shRNA-ASS1 (A) or shRNA-PYCR1 (C) in ECA109 and KYSE150 cells under low-O2 mircoenvironment. B, D Proliferation ability in OSCC cells transfected with control/shASS1 (B) and control/shPYCR1 (D). E–G Representative images of tumours in nude mice injected by control/shASS1/shPYCR1 (E). Weight (F) of subcutaneous tumour size. Protein levels of ASS1 and PYCR1 in control or shRNA-ASS1/PYCR1 xenografted tumours of nude mice (G). C = shRNA-Control sample; S = shRNA-ASS1/PYCR1 sample. H, I Proliferation ability was assessed in OSCC cells divided into groups control + DDP, shASS1(H)/shPYCR1(I) + DDP, shASS1(H)/shPYCR1(I) + Linsitinib + DDP. J, K, L Representative images of tumours in nude mice injected by control/shASS1/shPYCR1 combind with DDP (J). Weight (K) and volume (L) of subcutaneous tumour size. Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001