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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: CircFOXK2 promotes hepatocellular carcinoma progression and leads to a poor clinical prognosis via regulating the Warburg effect

Fig. 5

FOXK2-142aa positively correlates with the progression of HCC in vivo. A-B Huh7 cells stably receiving different treatments according to the group design (control, sh-circFOXK2 + FOXK2-142aa and sh-circFOXK2) were respectively used to subcutaneously inject into nude mice to establish xenograft tumors (n = 6 per group). A representative image of xenograft tumor formation in each group was obtained. C Analysis of the tumor volume among the three indicated groups measured three days. D The statistical analysis of the tumor weight among the three indicated groups. E Alterations in PCNA in xenograft tumors were investigated by IHC staining. (Bar = 20 μm). F The TUNEL assay was utilized to detect the apoptotic rate in xenograft tumors. G The expression of FOXK2-142aa, LDHA and p-LDHA in xenograft tumors in each group was detected by Western blot assay. The average intensity of the band was analyzed using β-actin as an internal control. Data were represented as means ± SD with at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001

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