Fig. 6

circFOXK2 acts as an miRNA sponge for miR-484 in HCC cells. A Map of circFOXK2 and its potential target miRNAs was depicted using Circlteractome. B qPCR analysis was conducted for the potential miRNAs pull-down by circFOXK2 in HepG2 and Huh7 cells. C Schematic illustrations exhibited the interactions of circFOXK2 with miR-484 by Arraystar's miRNA target prediction software. D The luciferase activity of wild-type and mutant circFOXK2 was detected by dual Renilla and firely luciferase reporter assays after co-transfected with either the miR-484 or control mimics in HepG2 and Huh7 cells. E RNA pulldown assays evaluated the circFOXK2 captured potential by biotinylated miR-484. F The locations of circFOXK2 (hsa_circ_0000817, red) and miR-484 (green) in HepG2 and Huh7 cells were detected using FISH. (magnification, 600 × ; scale bar, 100 μm). Data were represented as means ± SEM with at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001