Fig. 8

circFOXK2 regulates the Warburg effect in HCC cells via the axis of miR-484/Fis1-related mitochondrial fission. A-C Alterations in pyruvate production, lactate production and ATP production levels were detected in HepG2 and Huh7 cells. D-E The ECAR and OCR in HepG2 and Huh7 cells were measured. F The mRNA expression of Fis1 was assessed in HepG2 and Huh7 cells by qRT-PCR. G The protein expression of Fis1 was assessed in HepG2 and Huh7 cells by Western blotting. (H) Mitochondrial morphology was evaluated using a confocal microscope after MitoTracker Green staining. The ratio of the morphological changes of mitochondria per 100 cells in each group was analyzed according to the mitochondrial length. Data were represented as means ± SEM with at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001