Fig. 1

Colorectal tumorigenesis is related to neutrophil level and ARNT expression. A Quantitative PCR analysis of Arnt mRNA expression during neutrophil development from HSCs. The graphs summarize data from two independent experiments, each with six mice. B-C Quantitative PCR analysis (B) and western blot (C) of ARNT expression in neutrophils induced by GM-CSF for 4 days from BMs. The graphs summarize data from two independent experiments, each with six mice (B). Representative of two independent experiments (C). D-I Colorectal cancer induction for 80 days after the injection of azoxymethane (AOM) and dextran sulfate sodium salt (DSS). (D) Quantitative PCR analysis of Arnt mRNA expression in different subpopulations of tumor-infiltrating immune cells, including neutrophils (Neu; CD11b⁺Gr1⁺ cells), macrophages (Mac; CD11b⁺F4/80⁺ cells), DCs (CD11c⁺ cells), CD4⁺T cells (CD4⁺TCR⁺ cells), CD8⁺T cells (CD8⁺TCR⁺ cells) and B cells (CD19⁺ cells) isolated from tumors. Representative of three independent experiments with four mice. The correlations between the occurrence of tumors, the level of neutrophils and the expression of Arnt (E-G) and the correlations between the level of neutrophils, the expression of Arnt and the growth of tumors were analyzed (H-I), as indicated in the figure. The graph summarizes data from four independent experiments, each with five to six mice (E-I). ***P < 0.001, compared with the indicated groups