Fig. 2

Arnt^−/−enhanced the migration and functions of neutrophils. A Arnt^−/− enhanced the percentage of CD11b⁺Gr1⁺ neutrophils in BMs, blood, spleen and colon. Dot-plots present the representative data from flow cytometry analysis is shown (left), and the data are summarized (right). The graph summarizes data from two independent experiments with six mice per group. B Immunohistochemistry analysis of colon after staining with anti-Gr1 antibody. Scales bars, 50 μm. Original magnification, 400X. Representative of three independent experiments. C CXCR2 expression in neutrophils and dot-plots present the representative data from flow cytometry analysis is shown. Representative of three independent experiments. D Neutrophils from BMs were isolated and stimulated with LPS in vitro as indicated to detect neutrophil extracellular traps (NETs). Typical NET images are displayed (left), and the percentage and area of NETs are quantified (right). Scales bars, 20 μm (upper) and 50 μm (lower). Original magnification, 200X (upper) and 630X (lower). The graph summarizes data from three independent experiments with six mice per group. E The intracellular staining of TNFα and IL-10 in neutrophils was detected by flow cytometry. Dot-plots present the representative data from flow cytometry analysis is shown (left), and statistical results are shown (right). The graph summarizes data from three independent experiments with six mice per group. *, P < 0.05; **, P < 0.01 and ***P < 0.001, compared with the indicated groups