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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Aryl hydrocarbon receptor nuclear translocator limits the recruitment and function of regulatory neutrophils against colorectal cancer by regulating the gut microbiota

Fig. 5

Neutrophil Arnt−/−alters the gut microbiota in mice with colorectal cancer. Analysis of feces and fecal metabolites in WT and Arnt−/− mice. Feces were collected at the end of the experiment. Half of the fecal samples were used for DNA isolation and sequencing, and the other half were used for stimulating HT-29 cells. A Unweighted UniFrac-based PCoA plot based on all OTUs. B Total OTU sequences taxonomically assigned to bacterial genera. C Significantly different bacteria at the genus level between the WT and Arnt−/− groups. The graph summarizes data from three independent experiments with eleven or twelve mice per group (A-C). D RCTA indicated the effect of fecal metabolites on intestinal epithelial cells. Metabolites from cancer-induced mice obviously affected HT-29 cell adhesion and growth (green line and blue line), especially metabolites from Arnt−/− mice, which caused most HT-29 cells to die (blue line). Representative of three independent experiments. E The feces of WT and Arnt−/− mice were collected and intragastrically administered to WT mice every other day for induction of colorectal cancer by azoxymethane (AOM) injection and the addition of dextran sulfate sodium salt (DSS) to the drinking water for 80 days. The number of colonic tumors (left) and the tumor diameter (right) are summarized. The graph summarizes data from four independent experiments with sixteen mice per group. **, P < 0.01, compared with the indicated groups 

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