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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: L-kynurenine induces NK cell loss in gastric cancer microenvironment via promoting ferroptosis

Fig. 3

L-KYN induced NK cell ferroptosis in an AHR-independent way. A - D The proportion of FVD+ NK-92 or hNK cells after being treated with 200 μm L-KYN (A and B) or co-cultured with GES-1 or SGC-7901 cells (C and D) at the presence of the AHR inhibitor CH-223191 (1 μm) for 48 h, detected by flow cytometry. E The proportion of FVD+ NK-92 cells when treated with 200 μm L-KYN for 48 h, combining with 2 μm Fer-1, 1 μm Nec-1, 10 μm Z-VAD or 1 μm VX-765 respectively, detected by flow cytometry. F The flow cytometric detection for the mean fluorescence intensity (MFI) of MitoSox, LiperFluo and FerroOrange in NK-92 cells when treated with 200 μm L-KYN combined with or without 1 μm CH-223191 for 48 h. The pictures on the left were representative results, on which the numbers indicated the MFI of different samples. The MFI was normalized to the untreated + DMSO group. G The levels of MitoSox, LiperFluo and FerroOrange in primary hNK cells (hCD45+CD3CD56+) infiltrating in the tumor tissues formed by SGC-7901Con or SGC-7901IDO-KO cells in humanized mice, analyzed by flow cytometry. The MFI in the tumor-infiltrated hNK cells was normalized to that in the peripheral blood hNK cells (PB-hNK) of the same mice. All the results were replicated in 3 (B, D, G) or 4 (A, C, E, F) independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001

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