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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: L-kynurenine induces NK cell loss in gastric cancer microenvironment via promoting ferroptosis

Fig. 5

The GPX4-overexpressed NK-92 cells showed therapeutic potential towards GC. A The expression of GPX4 in NK-92GPX4-high or NK-92GPX4-con cells, measured by flow cymetry. The pictures on the left were representative results. B The percentage of ki-67+ subsets in NK-92GPX4-high or NK-92GPX4-con cells under normal conditions. C The percentage of FVD+ NK-92GPX4-high or NK-92GPX4-con cells when they were treated with or without L-KYN for 48 h, detected by flow cytometry. D and E The expression of IFN-γ(D), perforin and granzyme B (E) in NK-92GPX4-high or NK-92GPX4-con cells when stimulated with the cell stimulation cocktail (plus protein transport inhibitors) for 8 h. F The efficiency of NK-92GPX4-high or NK-92GPX4-con cells killing target cells when they were co-cultured for 6 h at an E: T ration of 1: 1. G The migration rate of NK-92GPX4-high or NK-92GPX4-con cells, measured by transwell assays. H The percentage of FVD+ NK-92GPX4-high or NK-92GPX4-con cells when they were co-cultured with or without SGC-7901 for 48 h. I The proportion of NK cells infiltrated in the SGC-7901-formed solid tumors in humanized hIL-15 NOG mice, after the mice being treated with PBS, 1 × 107 NK-92GPX4-high or NK-92GPX4-con cells. J The CDX tumors size, measured by a caliper at various time points. The pictures on the left were presentative results. All the results were replicated in 2 (I and J, n = 4 in per group) or 3 (A - H) independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001

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