Fig. 2

Loss of FBXW7 promotes the tumorigenic ability of ESCC. A Western blot analysis of FBXW7 expression in ESCC cell lines. B Western blot analysis of FBXW7 expression in KYSE70 and KYSE180 cells after sgRNA and transfected with plasmid in gFBXW7-1 cells. C Cell proliferation assay after FBXW7 knockout (gFBXW7-1 or gFBXW7-2) or rescued with FBXW7 plasmid or EV (empty vector) in gFBXW7-1 cells (FBXW7-RE). D Representative images and statistical plots of colony growth in KYSE70 and KYSE180 cells after FBXW7 knockout or rescued with FBXW7 plasmid in gFBXW7-1 cells. E Representative images and statistical plots of invasion assays in KYSE70 and KYSE180 cells after FBXW7 knockout or rescued with FBXW7 plasmid in gFBXW7-1 cells. F Representative images of tumors from the indicated group. G The weights of tumors from the indicated groups. H Tumor growth curve of tumors from the indicated groups. I Representative images and statistical plots of the lung metastatic nodules and the results of H&E staining are shown from the indicated group. Mean ± SEM are shown. *, p < 0.05, **, p < 0.01, ***, p < 0.001