Fig. 4

FBXW7 loss of function accelerates tumor progression via ERK phosphorylation. A Top 10 enriched pathways of differentially expressed genes following FBXW7 overexpression in KYSE70 cells. B GSEA analysis of RNA-seq upon negative control and FBXW7 overexpression in KYSE70 cells. C Western blot analysis of p-ERK, ERK, VEGFA, MMP3 and GAPDH in negative control and FBXW7 knockout KYSE70 and KYSE180 cells. D Real-time analysis of MMP3 and VEGFA mRNA in negative control and FBXW7 knockout KYSE70 and KYSE180 cells. E Western blot analysis of p-ERK, ERK, VEGFA, MMP3 and GAPDH in KYSE70 and KYSE180 cells in which FBXW7 was knocked out and transfected with different FBXW7 plasmids. F Real-time analysis of MMP3 and VEGFA mRNA in KYSE70 and KYSE180 cells in which FBXW7 was knocked out and transfected with different FBXW7 plasmids. G Western blot analysis of FBXW7, p-ERK, ERK, VEGFA, MMP3 and GAPDH in KYSE70 and KYSE180 cells treated with DMSO or the ERK inhibitor MK-8353. Means ± SEM are shown. *, p < 0.05, **, p < 0.01, ***, p < 0.001