Fig. 1
From: NAD pool as an antitumor target against cancer stem cells in head and neck cancer
Stem properties of CD10, CD184, CD19, CD133 and CD166 subpopulations in HNSCC cell lines. A) Measurement of CD10, CD184, CD19, CD133 and CD166 expression levels by RT‒qPCR in tumorspheres (TO) and total extracts (TE) of RPMI-2650 and Detroit-562 cells. B) Tumorsphere assay of positive and negative subpopulations isolated by cytometer. C) Tumor growth in vivo in xenografts (N = 6) from positive and negative subpopulations isolated by a cytometer. Representative images of tumor size are shown. The mean ± standard deviation is presented. Statistical analysis was performed with Student’s t test (*p < 0.05; **p < 0.01; ***p < 0.001). ND: not determined. D) Cellular plasticity of the different subpopulations in HNSCC. Analysis of the positive cells for CD10, CD184, CD19, CD133 and CD166 markers by FACS of the isolated positive and negative subpopulations after being separated by cytometry and cultured for 1 or 2 weeks depending on the cell line. Non selected cells were used as a control. ND: not determined