Fig. 5
From: TERRA G-quadruplex stabilization as a new therapeutic strategy for multiple myeloma
ATM pathway activation after hit 17 treatments in MM cell lines. Representative WB analysis of p-ATM (A) and γH2AX (B) in NCI-H929. C Representative images showing detection of γ-H2AX by immuno-FISH in hit 17 and control (DMSO) NCI-H929 cell lines. Interphase cells were stained with antiphospho-histone H2AX (green) and telomeric Cy3-labeled probe (red). Nuclei were counterstained with DAPI (blue). Scale bar, 10 μm. D WB analysis of p-CHEK1 and p-CHEK2 in both NCI-H929 and AMO-1 cells. Representative expression of CDKN1A (E) and CDKN1B (F) in NCI-H929 cell lines by RT-qPCR. **P <0.01; ***P<0.001