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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: METTL3 promotes chemoresistance in small cell lung cancer by inducing mitophagy

Fig. 2

METTL3 induces SCLC chemoresistance in vitro. A-B CCK-8 assays showed that knockdown of METTL3 decreased the chemotherapy IC50 values in chemoresistant H69AR and H446DDP cells. *P < 0.05, **P < 0.01. C-D Cell apoptosis and arrest were evaluated by flow cytometric analysis in METTL3-knockdown SCLC cells after treatment with chemotherapeutic drugs. E Apoptosis-related protein levels were measured by Western blotting following treatment with chemotherapeutic drugs in METTL3 knockdown cells. F-G CCK-8 assays showed that METTL3 overexpression increased the IC50 values of chemotherapeutic agents in chemosensitive H69 and H446 cells. *P < 0.05, **P < 0.01. H-I Cell apoptosis and arrest were evaluated by flow cytometry in METTL3-overexpressing SCLC cells following treatment with chemotherapeutic drugs. J Apoptosis-related proteins were measured by Western blotting following treatment with chemotherapeutic drugs in METTL3-overexpressing cells. K Western blot assays were performed to evaluate METTL3 expression after transfection with pcDNA3.1-METTL3 and pcDNA3.1-METTL3-mut in H69 cells. L CCK-8 assay showed that overexpression of METTL3 changed the IC50 value of chemotherapy drugs in SCLC cells. **P < 0.01; ns, not significant

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