Fig. 6
ALDOC and ENO2 knock down reduces the sphere-forming ability of H460 and MCF7 cells. A qRT-PCR analyses of ALDOC and ENO2 in H460 3D_SM, H460 3D_ FBSlow, MCF7 3D_SM, and MCF7 3D_ FBSlow upon ALDOC and ENO2 silencing alone or in combination. B Cell viability of H460 3D_SM, H460 3D_FBSlow, MCF7 3D_SM, and MCF7 3D_FBSlow upon ALDOC and ENO2 silencing alone or in combination assessed by Cell titer-Glo 3D assay and expressed as relative light unit (R.L.U). C Representative images and relative histograms of tumor spheroids morphology and diameter of H460 3D_SM, H460 3D_FBSlow, MCF7 3D_SM, and MCF7 3D_FBSlow upon ALDOC and ENO2 silencing alone or in combination. All the experiments were carried out in triplicate and results are presented as mean ± SD. p-value: * < 0.05, ** < 0.01, *** < 0.001. ns: not significant